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Preparation and characterization of Lip-Exo/Pae. (A) <t>Fluorescence</t> inverted <t>microscope</t> pictures of liposome-exosome fusion (liposomes in red, exosomes in green, Lip-Exo/Pae in yellow). (B) Schematic diagram of liposome and exosome fusion. (C) Appearance of Lip-Exo/Pae solution. (D) Tyndall effect of Lip-Exo/Pae colloidal solution. (E) TEM Image of Lip-Exo/Pae. TEM Image of Lip-Exo/Pae. Scale bars represent 200 nm. (F) Particle size distribution map of Lip-Exo/Pae. (G) Zeta potential and PDI of Lip-Exo/Pae. (H) In vitro dialysis release curves of each formulation group. (I) The release curves were fitted to each formulation group using the Weibull mathematical model. (J) ABTS clearance results for each group of formulations at varying concentrations. (K) The scavenging results of hydroxyl radicals by different concentrations of each formulation group. (L) Blood compatibility test results of Lip-Exo/Pae. (M) Cell viability after 24-h incubation of HT22 cells with varying concentrations of Lip-Exo/Pae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Preparation and characterization of Lip-Exo/Pae. (A) <t>Fluorescence</t> inverted <t>microscope</t> pictures of liposome-exosome fusion (liposomes in red, exosomes in green, Lip-Exo/Pae in yellow). (B) Schematic diagram of liposome and exosome fusion. (C) Appearance of Lip-Exo/Pae solution. (D) Tyndall effect of Lip-Exo/Pae colloidal solution. (E) TEM Image of Lip-Exo/Pae. TEM Image of Lip-Exo/Pae. Scale bars represent 200 nm. (F) Particle size distribution map of Lip-Exo/Pae. (G) Zeta potential and PDI of Lip-Exo/Pae. (H) In vitro dialysis release curves of each formulation group. (I) The release curves were fitted to each formulation group using the Weibull mathematical model. (J) ABTS clearance results for each group of formulations at varying concentrations. (K) The scavenging results of hydroxyl radicals by different concentrations of each formulation group. (L) Blood compatibility test results of Lip-Exo/Pae. (M) Cell viability after 24-h incubation of HT22 cells with varying concentrations of Lip-Exo/Pae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Preparation and characterization of Lip-Exo/Pae. (A) <t>Fluorescence</t> inverted <t>microscope</t> pictures of liposome-exosome fusion (liposomes in red, exosomes in green, Lip-Exo/Pae in yellow). (B) Schematic diagram of liposome and exosome fusion. (C) Appearance of Lip-Exo/Pae solution. (D) Tyndall effect of Lip-Exo/Pae colloidal solution. (E) TEM Image of Lip-Exo/Pae. TEM Image of Lip-Exo/Pae. Scale bars represent 200 nm. (F) Particle size distribution map of Lip-Exo/Pae. (G) Zeta potential and PDI of Lip-Exo/Pae. (H) In vitro dialysis release curves of each formulation group. (I) The release curves were fitted to each formulation group using the Weibull mathematical model. (J) ABTS clearance results for each group of formulations at varying concentrations. (K) The scavenging results of hydroxyl radicals by different concentrations of each formulation group. (L) Blood compatibility test results of Lip-Exo/Pae. (M) Cell viability after 24-h incubation of HT22 cells with varying concentrations of Lip-Exo/Pae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Preparation and characterization of Lip-Exo/Pae. (A) Fluorescence inverted microscope pictures of liposome-exosome fusion (liposomes in red, exosomes in green, Lip-Exo/Pae in yellow). (B) Schematic diagram of liposome and exosome fusion. (C) Appearance of Lip-Exo/Pae solution. (D) Tyndall effect of Lip-Exo/Pae colloidal solution. (E) TEM Image of Lip-Exo/Pae. TEM Image of Lip-Exo/Pae. Scale bars represent 200 nm. (F) Particle size distribution map of Lip-Exo/Pae. (G) Zeta potential and PDI of Lip-Exo/Pae. (H) In vitro dialysis release curves of each formulation group. (I) The release curves were fitted to each formulation group using the Weibull mathematical model. (J) ABTS clearance results for each group of formulations at varying concentrations. (K) The scavenging results of hydroxyl radicals by different concentrations of each formulation group. (L) Blood compatibility test results of Lip-Exo/Pae. (M) Cell viability after 24-h incubation of HT22 cells with varying concentrations of Lip-Exo/Pae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Bionic design based on liposome-exosome hybrid nanoparticles for synergistic delivery of paeonol to achieve neuroprotection and improvement of motor function in Parkinson's disease model mice

doi: 10.1016/j.mtbio.2026.102847

Figure Lengend Snippet: Preparation and characterization of Lip-Exo/Pae. (A) Fluorescence inverted microscope pictures of liposome-exosome fusion (liposomes in red, exosomes in green, Lip-Exo/Pae in yellow). (B) Schematic diagram of liposome and exosome fusion. (C) Appearance of Lip-Exo/Pae solution. (D) Tyndall effect of Lip-Exo/Pae colloidal solution. (E) TEM Image of Lip-Exo/Pae. TEM Image of Lip-Exo/Pae. Scale bars represent 200 nm. (F) Particle size distribution map of Lip-Exo/Pae. (G) Zeta potential and PDI of Lip-Exo/Pae. (H) In vitro dialysis release curves of each formulation group. (I) The release curves were fitted to each formulation group using the Weibull mathematical model. (J) ABTS clearance results for each group of formulations at varying concentrations. (K) The scavenging results of hydroxyl radicals by different concentrations of each formulation group. (L) Blood compatibility test results of Lip-Exo/Pae. (M) Cell viability after 24-h incubation of HT22 cells with varying concentrations of Lip-Exo/Pae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Finally, 10 μL Lip-Exo/Pae samples prepared using both methods were mounted on microscope slides, and fluorescence colocalization was assessed using a multi-channel scanning inverted fluorescence microscope (DMI 8, Leica, Germany) to determine membrane fusion.

Techniques: Fluorescence, Inverted Microscopy, Liposomes, Zeta Potential Analyzer, In Vitro, Formulation, Incubation